Effects of Melatonin on Synaptic Plasticity after Spinal Cord Injury in Rats

VernacularTitle:褪黑素对脊髓损伤大鼠突触可塑性的作用
Author: Yingli JING ; Xiaoye LIU ; Fan BAI ; Hao DONG ; Hui CHEN
Publication Type:Journal Article
Keywords: spinal cord injury; synaptic plasticity; melatonin; rats
From: Chinese Journal of Rehabilitation Theory and Practice 2016;22(7):774-778
CountryChina
Language:Chinese
Abstract: Objective To observe the effects of melatonin on synaptic plasticity impaired by spinal cord injury in rats. Methods A total of 54 female Sprague-Dawley rats were divided into sham group (n=18), control group (n=18) and melatonin group (n=18). Spinal cord inju-ry model was established with modified Allen's method at T10 (10 g from 25 mm height). The number of neurons and the expression of the Nissl body were detected with immunofluorescence and Nissl staining. The expression of neurofilament-200 (NF-200), brain-derived neuro-trophic factors (BDNF), Synapsin I and growth-associated protein-43 (GAP-43) was detected with Western blotting. Results Seven days af-ter injury, the number of motoneurons, the expression of Nissl body in motoneurons, and the expression of BDNF, Synapsin I and GAP-43 decreased in the control group compared with those in the sham group, and they increased in the melatonin group compared with those in the control group. Conclusion Melatonin can repair the impaired synaptic plasticity, which might promote the functional recovery after spi-nal cord injury.