Antibacterial activity and cytocompatibility of chitosan-nano-silver complex thermosensitive hydrogel
10.3969/j.issn.1005-1678.2016.06.16
- VernacularTitle:壳聚糖纳米银复合物基温敏水凝胶的抑菌性及细胞相容性研究
- Author:
Gaoqing LI
;
Qing SANG
;
Kai CUI
- Publication Type:Journal Article
- Keywords:
chitosan;
nano silver;
thermosensitive hydrogel;
antibacterial activity;
cytocompatibility
- From:
Chinese Journal of Biochemical Pharmaceutics
2016;36(6):63-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the antibacterial activity and cytocompatibility of chitosan-nano-silver complex thermosensitive hydrogel. Methods There were 4 groups: group A (containing 1 ×10-5 chitosan-nano-silver complex thermosensitive hydrogel), group B (containing 5 ×10-6 chitosan-nano-silver complex thermosensitive hydrogel) , group C ( containing 2 ×10-6 chitosan-nano-silver complex thermosensitive hydrogel) , group D ( chitosan thermosensitive hydrogel ) .The antibacterial activity of the samples against six kinds of gram-negative bacteria, one kind of gram-positive bacterium, three kinds of fungi were measured by bacteriostatic circle.The cytocompatibility of the extraction to NIH-3T3 cells was studied by SRB. Results The antibacterial activity enhanced with the increasing of nano silver concentration in chitosan-nano-silver complex thermosensitive hydrogel, whose antibacterial activity was better than chitosan thermosensitive hydrogel; its extraction has no cytotoxicity, thus showed good cytocompatibility. Conclusion The chitosan-nano-silver complex thermosensitive hydrogel is a potential novel wound dressing.
