MEF2C mediates inhibitory effect of microRNA-214 on cardiomyocyte hypertrophy
10.3969/j.issn.1000-4718.2016.08.001
- VernacularTitle:MEF2 C 介导 microRNA－214发挥抑制心肌细胞肥大的作用
- Author:
Chunmei TANG
;
Jiening ZHU
;
Wensi ZHU
;
Qiuxiong LIN
;
Zhiqin HU
;
Yongheng FU
;
Mengzhen ZHANG
;
Chunyu DENG
;
Honghong TAN
;
Shulin WU
;
Zhixin SHAN
- Publication Type:Journal Article
- Keywords:
Cardiac hypertrophy;
MicroRNA-214;
MEF2C;
Cardiomyocytes
- From:
Chinese Journal of Pathophysiology
2016;32(8):1345-1350
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of microRNA-214 ( miR-214) on cardiomyocyte hypertrophy and the expression of the potential target genes .METHODS:A cell model of hypertrophy was established based on angiotensin-Ⅱ( Ang-Ⅱ)-induced neonatal mouse ventricular cardiomyocytes ( NMVCs) .Dual luciferase reporter assay was performed to verify the interaction between miR-214 and the 3’ UTR of MEF2C.The expression of MEF2C and hypertrophy-related genes at mRNA and protein levels was determined by RT-qPCR and Western blot , respectively .RESULTS:The expression of ANP, ACTA1,β-MHC and miR-214 was markedly increased in Ang-Ⅱ-induced hypertrophic cardiomyocytes .Dual lu-ciferase reporter assay revealed that miR-214 interacted with the 3’ UTR of MEF2C, and miR-214 was verified to inhibit MEF2C expression at the transcriptional level .The protein expression of MEF2C was markedly increased in the hypertro-phic cardiomyocytes .Moreover, miR-214 mimic, in parallel to MEF2C siRNA, inhibited the expression of hypertrophy-re-lated genes in Ang-Ⅱ-induced NMVCs.CONCLUSION:MEF2C is a target gene of miR-214, which mediates the effect of miR-214 on attenuating cardiomyocyte hypertrophy .
