Effect of circRNA_000203 on fibrotic phenotypes in mouse cardiac fibro-blasts
10.3969/j.issn.1000-4718.2016.08.002
- VernacularTitle:circRNA＿000203对小鼠心肌成纤维细胞纤维化表型的影响
- Author:
Wensi ZHU
;
Chunmei TANG
;
Jiening ZHU
;
Qiuxiong LIN
;
Yongheng FU
;
Chunyu DENG
;
Hui YANG
;
Fang RAO
;
Shulin WU
;
Zhixin SHAN
- Publication Type:Journal Article
- Keywords:
Circular RNAs;
Cardiac fibrosis;
Extracellular matrix
- From:
Chinese Journal of Pathophysiology
2016;32(8):1351-1356
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine circular RNA (circRNA) profiles in the diabetic mouse myocardium , and to investigate the effect of circRNA_000203 on fibrotic phenotypes in cardiac fibroblasts .METHODS:Masson trichrome stai-ning was performed on the myocardium of the diabetic db /db mice and the non diabetic db/m control mice .circRNA ex-pression profile in the diabetic myocardium was detected by circRNAs microarray .The expression of circRNA_000203 was determined by real time fluorescence quantitative PCR ( RT-qPCR ) .Recombinant circRNA_000203 adenovirus was pre-pared for enforced the expression of circRNA_000203 in mouse cardiac fibroblasts.The expression of Col1a2, Col3a1andα-SMA was determined in circRNA_000203-modified cardiac fibroblasts , respectively .RESULTS:Masson trichrome stai-ning showed that fibrosis was increased in the diabetic mouse myocardium .The results of circRNA array detection revealed that circRNAs were dysregulated in the diabetic myocardium .circRNA_000203 was up-regulated in the diabetic myocardi-um.Significant over-expression of circRNA_000203 was achieved in the cardiac fibroblasts after infection with the recombi-nant circRNA_000203 adenovirus.The mRNA and protein expression of Col1a2, Col3a1 and α-SMA was significantly in-creased in the cardiac fibroblasts with over-expression of circRNA_000203.CONCLUSION:circRNA_000203 is up-regu-lated in the diabetic mouse myocardium .It has pro-fibrotic effect on the cardiac fibroblasts .
