Synergistic effect of insulin and selenium in combination on inhibiting myocardial apoptosis in rats with diabetic cardiomyopathy
10.3969/j.issn.1000-4718.2016.08.003
- VernacularTitle:胰岛素联合硒协同抗糖尿病心肌细胞凋亡的作用
- Author:
Tianjiao XU
;
Yong LIU
;
Ping LI
;
Xiaoli XU
;
Jurong ZENG
- Publication Type:Journal Article
- Keywords:
Insulin;
Selenium;
Diabetic cardiomyopathy;
Ku70;
Acetylation
- From:
Chinese Journal of Pathophysiology
2016;32(8):1357-1363
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of insulin and selenium in combination on the apoptosis and the ex-pression of Ku70, acetylated Ku70, Bax and cytochrome C in myocardial cells of the rats with diabetic cardiomyopathy (DCM), and to explore the mechanism of insulin and selenium in their synergistic anti-DCM effect.METHODS:SD rats (n=50) were randomly grouped into control, DCM, DCM with insulin treatment (DCM+In) group, DCM with selenium treatment (DCM+Se) group, and DCM with insulin and selenium combination treatment (DCM+In+Se) group.Mito-chondrial membrane potential ( MMP) was measured by flow cytometry .The cell apoptosis was observed by terminal-deoxy-nucleotidyl transferase mediated nick end labeling (TUNEL).The levels of Ku70, Bax and cytochrome C were examined by Western blot .The acetylation status of Ku 70 was detected by co-immunoprecipitation .RESULTS: The rats in DCM group showed marked cell apoptosis compared with the control rats .The levels of Ku70 and acetylated Ku70 declined sig-nificantly compared with control group .Bax significantly translocated from cytoplasm into mitochondria and cytochrome C translocated from mitochondria into cytoplasm compared with control group .Compared with DCM +In group or DCM +Se group, insulin and selenium in combination significantly inhibited the apoptosis , down-regulated Ku70 and acetylated Ku70 levels, and prevented Bax and cytochrome C translocation .CONCLUSION: Insulin and selenium synergistically inhibits myocardial apoptosis by regulating Ku 70 acetylation and inhibiting Bax translocation .
