Expression of miR-625-3p in colorectal carcinoma tissues and cells
10.3969/j.issn.1000-4718.2016.08.006
- VernacularTitle:miR－625－3p 在结肠癌组织和细胞中的表达
- Author:
Hailun ZHENG
;
Rui ZHAO
;
Dapeng LI
;
Qiangwu WANG
;
Jianchao WANG
;
Qizhi WANG
- Publication Type:Journal Article
- Keywords:
Colorectal carcinoma;
MicroRNA-625-3p;
Cell cycle;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2016;32(8):1376-1382
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of microRNA-625-3p ( miR-625-3p) in colorectal carcinoma ( CRC) and its underlying mechanism .METHODS:Quantitative real-time PCR was employed to detect the levels of miR-625-3p expression in different CRC cell lines , CRC tissues and pair-matched adjacent normal tissues .The relationships be-tween the expression levels of miR-625-3p and the patients’clinicopathological parameters were estimated .The effects of miR-625-3p on the apoptosis and the cell mitotic cycle of CRC cells were analyzed with propidium iodide staining and flow cytometry.The effect of miR-625-3p on the apoptosis-related proteins was analyzed by Western blot .RESULTS: The ex-pression level of miR-625-3p in the CRC tissues was higher than that in the pair-matched adjacent normal tissues ( P <0.05).The expression of miR-625-3p in the CRC tumor tissues was significantly correlated with the tumor infiltrative depth , TNM stage and distant metastasis (P<0.05).The expression levels of miR-625-3p in CRC SW620 cells were higher than that in SW480 cells.The CRC cell mitotic cycle was significantly inhibited and cell apoptosis was significantly promoted when the expression of miR-625-3p was inhibited (P<0.05).The expression of Bax protein didn’t change and the expres-sion of Bcl-2 protein increased after miR-625-3p mimics were transfected into CRC SW620 cells(P<0.05).CONCLU-SION:miR-625-3p may be a promising approach for the treatment of CRC by promoting cell proliferation and inhibiting apoptosis .
