Apelin-13 attenuates pressure overload-induced aortic adventitial remodeling and fibrosis in Sprague-Dawley rats
- Author:
Zhenzhou ZHANG
;
Yan ZHANG
;
Ran XU
;
Laijiang CHEN
;
Shujie GUO
;
Yingle XU
;
Qing CHANG
;
Pingjin GAO
;
Jiuchang ZHONG
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2016;32(8):1507-1507
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate regulatory roles of Apelin in adventitial remodeling and fibrosis in rats with transverse aortic constriction ( TAC) .METHODS:The male Sprague-Dawley rats with TAC were randomized to daily deliver either pyroglutamyl Apelin-13 ( 50μg/kg) or saline for 4 weeks.RESULTS:Histomorphometric analysis by HE and Masson Trichrome staining revealed increased medi -al and adventitial thicknesses , especially in the adventitia , in ascending aortas in rats with TAC when compared with the sham-operated rats.Downregulation of APJ receptor and elevations in phosphorylated mTOR and ERK 1/2 levels were observed in rats with TAC . There are marked increases in heart weight ( HW) , HW/body weight ratio , and aortic fibrosis in rats with TAC .The pressure over-load-mediated pathological adventitial remodeling was strikingly rescued by Apelin-13, associated with attenuation of aortic fibrosis and reduced mRNA expression of TGF-β1, fibronectin and collagen I .CONCLUSION:Our results demonstrate the importance of Apelin-13 in amelioration of aortic adventitial remodeling and fibrosis in rats with TAC via modulation of the mTOR /ERK signaling , thus indi-cating potential therapeutic strategies by enhancing Apelin /APJ action for preventing pressure overload-and fibrosis-associated cardio-vascular disorders .
