Knockdown of CMTM3 promotes migration and invasion of PC3 cell in vitro
10.3969/j.issn.1671-167X.2016.04.005
- VernacularTitle:敲减 CMTM3增强前列腺癌细胞系 PC3迁移与侵袭能力
- Author:
Fengzhan HU
;
Wanqiong YUAN
;
Xiaolin WANG
;
Caipeng QIN
;
Zhengzuo SHENG
;
Yiqing DU
;
Huaqi YIN
;
Tao XU
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
CMTM3;
Gene knockdown techniques;
Cell migration assays;
In vitro
- From:
Journal of Peking University(Health Sciences)
2016;48(4):594-597
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the change of biological characteristics after stable knockdown of CKLF-like MARVEL transmembrane domain containing 3 (CMTM3)expression in PC3 by lentivirus shRNA and to reveal new therapeutic targets.Methods:The research includes two groups:sh393 is the experimental group in which CMTM3 is knocked down in PC3 cell line;shN is the control group in which CMTM3 is negatively knocked down.The expression of CMTM3 was detected by Western blot.The mi-gration ability of PC3 after stable knockdown was detected by Transwell and Wound healing assay.The invasion ability of PC3 was detected by Matrigel assay.Results were obtained from at least three indivi-dual experiments.Results:The expression of CMTM3 in sh393 group is significant lower than shN group (0.004 0 ±0.000 4 vs.0.490 0 ±0.055 7,P <0.001)detected by Western blot.It also had statistical significance in Matrigel assays (248.6 ±4.5 vs.113.0 ±3.3),Transwell (203.6 ±1.9 vs.103.0 ± 1.2)and Wound healing assays (95.0 ±2.9 vs.33.0 ±1.5)that knockdown of CMTM3 promoted mi-gration,and invasion of PC3 cells in vitro (P <0.001).Conclusion:Negative correlation exists between the stable knockdown of CMTM3 and change of biological characteristics in PC3 cells,and knocking down CMTM3 affects migration,and invasion ability in PC3 cells.
