Research progress in signal amplification-based methods for microRNA detection
10.3760/cma.j.issn.1009-9158.2016.07.019
- VernacularTitle:基于信号放大技术的 miRNA 检测方法的研究进展
- Author:
Jiamin ZHAO
;
Weifeng LYU
;
Lei ZHENG
- Publication Type:Journal Article
- Keywords:
Ribonucleic acid;
Detection method;
Signal amplification
- From:
Chinese Journal of Laboratory Medicine
2016;39(7):548-551
- CountryChina
- Language:Chinese
-
Abstract:
MicroRNA (miRNA) are a class of endogenous single -stranded non-coding RNA, which regulate gene expression post-transcriptionally by combining with the target mRNA and play a vital role in biological and pathological processes including the growth of organism , metabolic regulation, disease prediction and intervention.Thus miRNA detection is of considerable significance in disease diagnosis and the research of miRNA function.Because of the restriction factors about miRNA itself such as short sequence, low abundance and highly homologous , traditional methods for miRNA detection cannot meet the current demands due to the limitations like unsatisfactory sensitivity and complicated operation .This review summarizes the newly development about signal amplification -based methods for miRNA, including the advantages and limitations of all kinds of novel methods , and highlights the future trends as well.
