Factors accounting for HIV antibody test false positive results in patients with rheumatoid arthritis
10.3760/cma.j.issn.1009-9158.2016.07.012
- VernacularTitle:类风湿性关节炎患者 HIV 抗体检测假阳性结果影响因素的分析
- Author:
Yunchun LI
;
Li ZHONG
;
Yue WANG
;
Fan YANG
;
Zhongjun FANG
;
Liumei DING
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Human immunodeficiency virus;
False positive reactions;
Autoantibodies;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Laboratory Medicine
2016;39(7):522-525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate if immunological factors associated with the false HIV screening test results in RA.Methods Subjects who attended the Rheumatology Outpatient Clinic -Internal Medicine Unit of Guanghua Integrative Hospital , from October 2013 to October 2014, who met the American College of Rheumatology /European League Against Rheumatism Criteria for RA were recruited for the study . 100 subjects with RA were recruited.Each patient underwent clinical examination and blood sampling for assessment of serum HIV screening test and Rheumatoid factors ( RF-IgA, -IgG, -IgM) and anti-cyclic citrullinated protein antibodies (anti-CCP) were purified from the plasma and detected by ELISA , Samples were collected and processed using standard protocols and were stored in the same freezer before analysis . RA patients were divided into two groups based on the titters of RF and anti -CCP:RF <18 U/ml/anti-CCP <25 U/ml group and RF >300 U/ml /anti-CCP >500 U/ml group.HIV screening tests were determined by three methods: ELISA、Immuno-colloidal Golden Method and ECLIA.The positive results were confirmed by the Changning Centers for Disease Control , Shanghai through western -blotting test.Results 100 samples detected by ELISA and Immuno-colloidal Golden Method were given negative results , 16 positive results existed in ECLIA group.There were1,12,3 positive cases in RF-IgM <18 U/ml, RF-IgM and RF-IgG >300 U/ml group(2.7%,32.4%,13.6%;P <0.01).In anti-CCP <25 RU/ml and >500 RU/ml groups there were 2 and 4 positive results(4.7%,24.6%;P <0.01).Conclusions Different HIV screening test methods would give different results , according to operation requirement using second method to determine the HIV screening result.HIV False-positivity was associated with the titers of anti -CCP and RF in RA.
