Determination of Nine Residual Solvents in Grape Seed Extract by Headspace Gas Chromatography
10.3870/j.issn.1004-0781.2016.09.022
- VernacularTitle:顶空气相色谱法测定葡萄籽提取物中9种残留溶剂
- Author:
Yiming LI
;
Xiaojin CHEN
;
Shaolei LIU
;
Jianyun YANG
;
Bingkun XIAO
;
Rongqing HUANG
- Publication Type:Journal Article
- Keywords:
Grape seed extract;
Chromatography,gas;
Solvent,residual
- From:
Herald of Medicine
2016;35(9):994-997
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a headspace gas chromatography method for determination of nine residual solvents in grape seed extract. Methods The residual solvents in grape seed extract were separated on DB-WAX column (30 m× 0.45 mm, 0.85 μm) with an FID detector; the injector temperature and the detector temperature was set at 220 ℃ and 250 ℃ , respectively; the chapiter pressure was 27.58 kPa; the containers of headspace injector were in equilibrium for 30 min at 80 ℃ ;the N, N-dimethylformamide was used as the solvent. Results The detected solvents were well separated. Good linear relationship of the benzene and ethanol was obtained within the range of 0.04-0.3,100-750 μg?mL-1 , respectively. Good linear relationships of the hexane, o-xylene, m-xylene, p-xylene, styrene, toluene and 1,2-diethyl benzene solvents were obtained within the range of 0.4-3.0 μg?mL-1 (r≥0.995 8), respectively. The average recoveries (n = 3) of the nine solvents were 96.35%,97.08%,97.31%,89.93%,92.35%,90.65%,88.56%,93.84%,86.51% and the RSDs were 4.38%,2.16%,3.49%, 4.19%,4.80%,4. 83%,4. 70%,5. 00%,4. 39%, respectively. Conclusion The established method is simple, rapid and accurate, which can be used for the determination of residual solvents in grape seed extract and can simultaneously provide scientific basic for quality control.
