A Simultaneous Determination of Five Components in Radix et Rhizoma Salviae Miltiorrhizae by RP-HPLC
10.3870/j.issn.1004-0781.2016.09.023
- VernacularTitle:反相高效液相色谱法同时测定丹参中5种成分含量
- Author:
Xiaoyan JIANG
;
Lin ZHANG
;
Zhihua DOU
;
Xia CHEN
;
Zhengyu WANG
- Publication Type:Journal Article
- Keywords:
Radix et Rhizoma Salviaemiltiorrhizae;
Chromatogrophy,reversed phase-high performance liquid;
Tanshinol;
Rosmarinic acid;
Salviamolic acid B;
Clyptotanshinon;
Tanshinone II A;
Content determination
- From:
Herald of Medicine
2016;35(9):997-1000
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for simultaneous determination of three hydrophilic components and two lipophilic components in Radix et Rhizoma Salviae Miltiorrhizae. Methods The RP-HPLC method was performed by using a Welchrom C18 column(250 mm×4.6 mm,5 μm)with a mobile phase of acetonitrile (A)-0.1%phosphoric acid(B). The gradient elution program was as follows:0-15 min,10%→12%A;-35 min,12%→20%A;-45 min,20%→60%A;-65 min,60%→65%A;-80 min,65%→80%A;-90 min,10%A. The flow rate was kept at 1.0 mL?min-1 . The detection wavelength was set at 280 nm. The column temperature was 30 ℃ . Results A good linearity was obtained over 0.059 5-2.380 0 μg for tanshinol, 0.346 0-13. 840 0 μg for rosmarinic acid, 0. 656 0 - 26. 240 0 μg for salviamolic acid B, 0. 420 0 - 16.800 0 μg for cryptotanshinone and 0.414 0- 16.560 0 μg for tanshinoneIIA, respectively ( r = 0.999 9). The average recovery rates were between 98.69%-100.91% with RSD less than 1.2%(n = 6). Conclusion The method is rapid, accurate, credible and repeatable, and can provide basis for the quality control of Radix et Rhizoma Salviae Miltiorrhiza.
