Effects of Triptolide on Proliferation and Apoptosis of Cyst-lining Epithelial Cells of Polycystic Kidney Disase
10.3870/j.issn.1004-0781.2016.09.003
- VernacularTitle:雷公藤内酯醇对多囊肾囊肿衬里上皮细胞增殖和凋亡的影响?
- Author:
Huanrong YANG
;
Shumei YANG
;
Guangxin WANG
;
Fanjie MENG
;
Shufang CAI
;
Wenbin WU
;
Shijun WANG
- Publication Type:Journal Article
- Keywords:
Triptolide;
Polycystic kidney disease;
Epithelial cell,cyst-lining;
Cell proliferation;
Cell cycle;
Apoptosis
- From:
Herald of Medicine
2016;35(9):920-924
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of triptolide (TP) on proliferation and apoptosis of cyst-lining epithelial cells with autosomal dominant polycystic kidney disease ( ADPKD). Methods Primary cultured cyst-lining epithelial cells were treated with TP at different concentrations for 12 h,24 h,48 h and 72 h, respectively.The proliferation activity of the cells was evaluated by Brdu assay. The cell cycle distribution was determined by flow cytometry. The apoptotic and apoptotic ratio were determined by FITC-AnnexinV binding/ PI. The morphological changes of cyst-lining epithelial cells were observed under transmission electron microscope. Results TP significantly inhibited the proliferation of cyst-lining epithelial cells and induced apoptosis in a dose- (10-40 ng?mL-1 )and time-dependent(12-48 h) manner. Typical ultrastructural changes of apoptotic cells were observed under electron microscope. Conclusion TP significantly inhibited the proliferation of cyst-lining epithelial cells and induced the apoptosis of cyst-lining epithelial cells, thus inhibited cyst forming and delayed cyst developing. The mechanism may involve several targets and pathways.
