Potent rapid antidepressant effects of effective fraction of GJ-PE and up-regulation of BDNF and TrkB
10.3969/j.issn.1001-1978.2016.09.008
- VernacularTitle:栀子石油醚有效部位的快速抗抑郁潜力与BDNF、p-eEF2的表达上调相关
- Author:
Li REN
;
Weiwei TAO
;
Yi CHAI
;
Shouxue LI
;
Xing WANG
;
Yuxuan ZHANG
;
Li CHEN
;
Baomei XIA
;
Haoxing WU
;
Gang CHEN
- Publication Type:Journal Article
- Keywords:
Gardenia jasminoides Ellis;
petroleum e-ther fraction;
rapid antidepressant;
BDNF;
p-eEF2;
ketamine
- From:
Chinese Pharmacological Bulletin
2016;32(9):1224-1230
- CountryChina
- Language:Chinese
-
Abstract:
Aim To identify whether the petroleum e-ther fraction of Gardenia jasminoides Ellis ( GJ-PE ) could effetive exhibit a rapid antidepressant effect and also to investigate the biological mechanism. Methods Tail suspension test ( TST ) , forced swimming test ( FST ) and novelty suppressed-feeding ( NSF ) were used to screen the rapid antidepressant potential of ef-fective fractions of GJ-PE in KM mice at 24 h post a single administration. Tail suspension test ( TST) was also used at 30 min and forced swimming test ( FST ) was used at 2 h to test the initial onset time of effective fractions of GJ-PE in KM mice. Western blot was per-formed to examine the expression of BDNF and p-eEF2 in hippocampus of KM mice at 2 h and 24 h. Results An acute administration of GJ-PE1 decreased the im-mobility time of KM mice in FST at 2 h and 24 h and decreased the latency time in NSF at 24 h. GJ-PE3 de-creased the latency time in NSF at 24 h. GJ-PE4 in-creased the unit food consumption in NSF at 24 h. At 2 h post a single GJ-PE1 treatment, the expression of BDNF was significantly up-regulated while the expres-sion of p-eEF2 was significantly down-regulated. At 24 h post a single GJ-PE1 treatment, the expression of BDNF was significantly down-regulated while p-eEF2 expression was significantly up-regulated. Conclusion GJ-PE1 has the most significant rapid antidepressant potential among the four fractions of GJ-PE. The effec-tive time of GJ-PE1 is 2 h after drug treatment. The mechanism of the rapid antidepressant effect of GJ-PE1 at 2 h is related to the up-regulation of BDNF and down-regulation of p-eEF2 . GJ-PE3 and GJ-PE4 also have some features of rapid antidepressants. GJ-PE2 doesn′t have the rapid antidepressant potential.
