Inhibition of lipid peroxidation and protective effects on diabetic mice of extracts from Coreopsis tinctoria Nutt. in Xinjiang
10.3969/j.issn.1001-1978.2016.09.017
- VernacularTitle:新疆两色金鸡菊提取物抗脂质过氧化及对糖尿病小鼠的保护作用
- Author:
Ting ZHANG
;
Huifang LI
;
Bo LYU
;
Hui TANG
;
Xincheng YAO
;
Qingguang LIU
- Publication Type:Journal Article
- Keywords:
Coreopsis tinctoria Nutt.;
n-butanol ex-tract;
lipid peroxidation;
streptozotocin;
biochemical indexes;
organ coefficient;
diabetic mice
- From:
Chinese Pharmacological Bulletin
2016;32(9):1272-1278
- CountryChina
- Language:Chinese
-
Abstract:
Aim To compare the inhibition of lipid peroxidation of ethyl acetate extract( EAE) and n-buta-nol( BE) extract from Coreopsis tinctoria Nutt. in vitro. To investigate the parameters such as body weight, bio-chemical indexes in plasma, and viscera indexes on type 2 diabetes mice by intraperitoneal injection of streptozotocin ( STZ ) . Methods The extracts were prepared by response surface methodology. The ex-tracts were suspended in distilled water and defatted with petroleum ether. The aqueous layer was succes-sively extracted with ethyl acetate and n-butanol. The inhibition of lipid peroxidation activity was determined by thiobarbituric acid method. The effects of extract BE on diabetic mice were observed at the dosage of 0. 2,0. 4,0. 8 g·kg-1 ( ig) for 4 weeks. The parame-ters were observed such as weight of body changes, or-gan coefficients of liver, pancreas and kidney, bio-chemical indexes in plasma and viscera pathological sections. Results In the linoleic acid reaction system, the SC50 value of the EAE and BE was ( 443. 96 ± 11. 24) mg·L-1, (840. 29 ± 16. 38) mg·L-1, re-spectively, and that in rat liver homogenate was (23. 59 ± 3. 67 ) mg · L-1 , ( 60. 37 ± 4. 27 ) mg · L-1 , respectively. Compared with diabetic model group, BE could significantly improve the trend of weight loss, and increase viscera indexes. The patho-logical sections showed that BE had the recovery and improvement effects on the damage of liver, pancreas and kidney. Conclusions The extracts of C. tinctoria have a certain anti-lipid peroxidation activity in vitro. And BE has a certain capacity to improve and repair damaged organs for DM mice.
