Alizarin activates large-conductance Ca2+-activated K+ channels on renal interlobar arterial vascular smooth muscle cells in Wistar rat
10.3969/j.issn.1001-1978.2016.09.015
- VernacularTitle:茜草素激活Wistar大鼠肾叶间动脉平滑肌细胞大电导钙激活钾通道
- Author:
Qiong HE
;
Jianing WANG
;
Yanjie GAN
;
Yuanyuan WANG
;
Chuanlin ZHANG
- Publication Type:Journal Article
- Keywords:
alizarin;
patch clamp recording;
renal in-terlobar arterial;
BKCa;
Ca2+;
smooth musclecell
- From:
Chinese Pharmacological Bulletin
2016;32(9):1264-1267
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investage the regulatory effect of alizarin on renal interlobar arterial smooth muscle cells. Method The effect of alizarin on BKCa channels was observed by whole-cell patch clamp recording tech-nique. Results Selective BKCa channel blocker ibTX inhibited alizarin-induced outward current(P<0. 05);single smooth muscle cells were incubated in extracel-lular fluid with no Ca2+ 30 minutes, selective L-Ca2+channel blocker nimodipine, selective calcium ion che-lating agent BAPTA-AM and ryanodine inhibited the a-lizarin-induced BKCa channels current, too ( P <0. 05 ) . Conclusion Alizarin relaxes renales interlobar arterial smooth muscle via activation of L-Ca2+ channel firstly, which lead to Ca2+ flowed into cytoplam, and rising of Ca2+ in cytoplam ryanodine receptor indirect-ly, and BKCa is activated at last.
