Effect of HSP70 on HBV replication
10.3969/j.issn.1000-4718.2016.08.014
- VernacularTitle:热休克蛋白70对 HBV 复制的影响
- Author:
Hongzhong ZHOU
;
Bo LIU
;
Jihua REN
;
Nana TAO
;
Xiang CHEN
;
Wanyu LI
;
Juan CHEN
- Publication Type:Journal Article
- Keywords:
Heat shock protein 70;
HBV
- From:
Chinese Journal of Pathophysiology
2016;32(8):1425-1429
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the role of heat shock protein 70(HSP70)in hepatitis B virus (HBV) replica-tion.METHODS:The effect of HBV replication on the expression of HSP 70 was analyzed by RT-qPCR.The overexpres-sion efficiency of HSP70 was confirmed by Western blot .The effect of HSP70 overexpression on HBV DNA replicative in-termediates was analyzed by RT-qPCR and Southern blot .The effects of HSP70 overexpression on the expression level of HBV 3.5 kb mRNA and HBV core protein were measured by RT-qPCR and Western blot, respectively.The Effect of HSP70 overexpression on HBV promoter activity was detected by dual luciferase reporter system .RESULTS: The mRNA levels of HSP70 were inhibited by HBV replication .Overexpression of HSP70 repressed the expression of HBV DNA repli-cative intermediates, 3.5 kb mRNA and core protein, as well as HBV core promoter activity .CONCLUSION:HBV rep-lication inhibits the expression of HSP70.Overexpression of HSP70 represses HBV replication.These data suggest that HSP70 repressed HBV replication by inhibiting HBV core promoter activity .
