Effects of all-trans retinoic acid on sensitivity of gastric cancer cells to ra-diotherapy
10.3969/j.issn.1000-4718.2016.08.017
- VernacularTitle:全反式维甲酸增加胃癌细胞对放射的敏感性
- Author:
Yanping WANG
;
Xianqun ZHAO
;
Xiangdong ZHANG
;
Wei XU
;
Xiaohui XIANG
- Publication Type:Journal Article
- Keywords:
All-trans retinoic acid;
Radiotherapy;
Gastric cancer cell SGC-7901;
Sensitivity
- From:
Chinese Journal of Pathophysiology
2016;32(8):1440-1444
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of all-trans retinoic acid ( ATRA) on the viability of gastric cancer cell SGC-7901 and the sensitivity to radiotherapy .METHODS:MTT assay was used to examine the cell viability .Radio-sensitivity and cell cycle were determined by colony formation assay and flow cytometry , respectively .The mRNA levels of Bax, Bcl-2, survivin and NF-κB in the cells were measured by RT-qPCR.RESULTS: ATRA inhibited the viability of SGC-7901 cells in a concentration-dependent manner .The maximal inhibition was at concentration of 8 μmol/L.Colony formation assay revealed that the combination of ATRA with X-ray treatment significantly reduced the values of D 0 and Dq, and shifted down the fitting survival curve , as compared with radiotherapy alone .Moreover , ATAR markedly decreased the percentage of G2/M phase in the SGC-7901 cells (P<0.05).In addition, following ATRA treatment, the mRNA levels of Bcl-2 and survivin were decreased (P<0.05), whereas the mRNA levels of Bax and NF-κB were increased (P<0.05). CONCLUSION:ATRA enhances the sensitivity of SGC-7901 cells to radiotherapy , inhibits G2/M arrest and regulates the mRNA expression of Bax , Bcl-2, survivin and NF-κB.
