Protective Effect of Pyrola decorata H. Andres on β-amyloid Protein- induced Cytotoxicity in PC12 Cells
10.3870/j.issn.1004-0781.2016.09.009
- VernacularTitle:鹿衔草对β淀粉样蛋白诱导损伤的PC12细胞的保护作用?
- Author:
Xiliang YANG
;
Zonghao LIN
;
Zhizheng SHENG
;
Fang YANG
;
Bing XU
;
Kuojian LU
;
Qiong YUAN
;
Xiamin HU
- Publication Type:Journal Article
- Keywords:
Pyrola decorata;
Ursolic acid;
β amyloid protein;
PC12 cell
- From:
Herald of Medicine
2016;35(9):947-951
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate protective activity against Aβ25-35-induced cytotoxicity in PC12 cells of different extracts and ursolic acid, which were isolated from pyrola decorata. Methods Aβ25-35-induced cytotoxicity in PC12 cells was established as the model in vitro. The cultured PC12 cells were divided into blank control group, DMSO control group, model control group, different extract groups of pyrola decorate and ursolic acid(UA) group. The different extract groups included ether extract (PE), acetidin extract (AE), n-butanol extract (BE), the water extract (WE), 50% ethanol extract (HEE). MTT assay was used to test the optimum concentration, and the number of viable cells in culture medium was measured by ELISA at 490 nm wavelength. Results The cell viabilities in different extracts groups(PE, AE, BE, WE, HEE) were respectively 89.3%, 77.2%, 79. 2%, 75. 1% ,74. 0% at the concentration of 5. 0 mg ? mL-1 . Moreover, ursolic acid showed the best neuroprotective activity (88.9%) at the concentration of 500 μg?L-1 . Compared with model control group, the survival rate of each group was remarkably increased, and the protective activities of PE and UA were more significant among them. Conclusion Different polar extracts of pyrola decorata and isolated ursolic acid have neuroprotective effects on Aβ25-35-induced cytotoxicity in PC12 cells in certain degrees.
