Effect of glucagon-like peptide-1 receptor agonists on growth and energy metabolism in human medullary thyroid cancer cells
10.19401/j.cnki.1007-3639.2016.06.002
- VernacularTitle:胰高糖素样肽-1受体激动剂对人甲状腺髓样癌细胞生长和能量代谢的影响
- Author:
Hong WANG
;
Siliang ZHANG
;
Haixia GUAN
- Publication Type:Journal Article
- Keywords:
Medullary thyroid cancer;
Exenatide;
Liraglutide;
Energy metabolism
- From:
China Oncology
2016;26(6):487-491
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:This study aimed to investigate the effect of glucagon-like peptide-1 receptor agonists on proliferation, secretion of calcitonin and energy metabolism of medullary thyroid cancer (MTC) cell.Methods:The MTC cell line (TT) was culturedin vitro. After treatment with exenatide and liraglutide (0, 1, 10 and 100 nmol/L) for 24, 48 and 72 h, the proliferation of TT was analyzed by CCK-8 kit, the calcitonin was measured by calcitonin assay kits, and the energy metabolism of TT was measured by Seahorse XF instrument.Results:When compared with control group, neither exenatide nor liraglutide had effects on proliferation of TT (P>0.05); the calcitonin levels did not change signiifcantly after treatment with GLP-1 receptor agonists (P>0.05). Exenatide and liraglutide did not alter glycolysis and mitochondrial respiration in TT cells in a dose- and time-dependent manner.Conclusion:GLP-1 receptor agonists have no effect on the development of TT. Further collection of the safety data of exenatide and liraglutide on thyroid is still needed.
