Prenatal exposure to lipopolysaccharide results in lipid metabolism and FAT/CD36 expression in mice offspring
10.3969/j.issn.1001-1978.2016.08.010
- VernacularTitle:孕期炎症刺激对子代小鼠脂质代谢及 FAT／CD36表达的影响
- Author:
Shugang QIN
;
Xin CHEN
;
Yi JIA
;
Jianzhi ZHOU
;
Min SU
;
Xiaohui LI
- Publication Type:Journal Article
- Keywords:
prenatal exposure to lipopolysaccharide;
FAT/CD36;
FFA;
TG;
lipometabolic disorder;
obesity
- From:
Chinese Pharmacological Bulletin
2016;32(8):1080-1085
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of prenatal expo-sure to lipopolysaccharide ( LPS ) on lipid metabolism in mice offspring from the starting point of FAT/CD36 expression.Methods 8-week old C57 mice mated 2∶1, then they were caged separately , marked as preg-nancy 0 d.The pregnant mice were given single intrap-eritoneal injection of 75 μg? kg -1 LPS, and the con-trol received injections of 0.2 mL saline .The perirenal adipose of female mice and epididymis adipose of male mice were collected in 4 w,8 w,12 w,respectively. The weight of visceral adipose tissue and the free fatty acid( FFA) and triglyceride ( TG) of adipose tissue and FAT/CD36 of offspring mice were quantitated .Results The body weight of offspring of LPS group was also significantly higher than that of NS group , and LPS group offspring displayed increased adipose tissue wet weights , the expression of TG and FFA was increased in LPS group compared with NS .Especially , prenatal exposure to inflammatory stimulation resulted in marked increase of FAT/CD36 and abnormal adipocyte development .Conclusions Inflammation induced by prenatal exposure to LPS results in increased body weight , adipose coefficient and FAT/CD36 that might develop into obesity in adult mice .These results are relevant in that anomalous local adipose tissue and FAT/CD36 regulation may be an important mechanism underlying obesity .
