Hemostatic effect and mechanism of a non-polysaccharide fraction of Bletilla striata
10.3969/j.issn.1001-1978.2016.08.018
- VernacularTitle:白及非多糖组分的止血作用及其机制的初步研究
- Author:
Feifei ZHAO
;
Xin YANG
;
Dan XU
;
Li DONG
;
Jing LI
;
Yonglin WANG
;
Shanggao LIAO
- Publication Type:Journal Article
- Keywords:
Bletilla striata;
non-polysaccharide frac-tion;
hemostasis;
platelet aggregation;
bleeding time;
coagulation time;
mechanism
- From:
Chinese Pharmacological Bulletin
2016;32(8):1121-1126
- CountryChina
- Language:Chinese
-
Abstract:
Aim To understand the hemostatic effect of a non-polysaccharide fraction of Bletilla striata ( BS-80EE) and to clarify its mechanism of action .Methods The non-polysaccharide fraction ( BS-80 EE ) was prepared by passing the 95%ethanol extract of Bletilla striata through a D101 macroporous resin column elu-ted first with water and then with 80%ethanol.Bleed-ing time ( BT ) and clotting time ( CT ) of heparinized mice were employed as indicators for evaluating the he-mostatic effect of BS-80EE.The mechanism of action was investigated through observing the effect of BS-80 EE on platelet aggregation induced by adenosine diphosphate ( ADP) in rats with nephelometry and tes-ting the effect of BS-80EE on the thrombin time(TT), prothrombin time(PT), activated partial thromboplas-tin time(APTT), fibrinogen(FIB), P-selectin(P-S), thrombin-antithrombin complex ( TAT ) , D-dimer ( D-D) and plasminogen activator inhibitor-1 ( PAI-1 ) . Results BS-80 EE significantly shortened the CT and BT( P<0.01 or 0.05 ) of heparin mice in a dose-de-pendent manner; groups of all doses significantly re-duced the rat TT ( P <0.01 or 0.05 ) , and the high-dose group significantly increased the FIB content ( P<0.05); the mid-dose group and high-dose groups of BS-80EE significantly increased the contents of P-S, TAT and PAI-1 , while reduced the D-D production in rats ( P <0.01 ); although dose-dependent reductions of APTT and PT were observed for each treatment-group, no significance was observed .Conclusion BS-80EE possess pronounced hemostatic effect by promo-ting platelet aggregation and coagulation .
