Preparation of simulated stool specimens for proficiency testing scheme on the detection of Salmonella and Shigella
10.3760/cma.j.issn.0254-5101.2016.07.005
- VernacularTitle:沙门菌和志贺菌实验室能力比对验证的模拟粪便标本的研制和评估
- Author:
Dongmei HE
;
Bixia KE
;
Hailing TAN
;
Bosheng LI
;
Honghui ZENG
;
Tong YANG
;
Changwen KE
;
Yuheng LIANG
;
Meizhen LIU
- Publication Type:Journal Article
- Keywords:
Proficiency testing;
Salmonella;
Shigella;
Escherichia coli
- From:
Chinese Journal of Microbiology and Immunology
2016;36(7):506-511
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare simulated stool specimens for proficiency testing ( PT) by mix-ing lentils with Salmonella, Shigella and Escherichia coli strains and to establish an assessment scheme for the detection of Salmonella and Shigella in clinical samples. Methods Salmonella, Shigella and Escherich-ia coli strains were respectively spiked to lentils in Cary-Blair transport medium to create simulated stool specimens. Various ratios of Escherichia coli to Salmonella strains were spiked to lentils to prepare mixed simulated stool specimens. The accuracy and stability of prepared stool samples for PT were tested in-house. Results of sample detection were collected from participating laboratories for further external quality assess-ment. Results The Escherichia coli and Salmonella strains mixed at ratios of 100 ∶ 1 to 106 ∶ 1 could be ef-ficiently isolated from the media. Enrichment was needed in order to effectively isolate Salmonella strains from the media when the ratios of Escherichia coli to Salmonella strains were 104 ∶ 1 to 106 ∶ 1. Of the16 participating laboratories, 14 laboratories (87. 5%) received a grade of“satisfactory” and the other 2 labo-ratories (12. 5%) received a grade of “mainly satisfactory”. Conclusion The simulated stool specimens and the PT procedures designed in this study were suitable for proficiency testing program on the detection of Salmonella, Shigella and other similar microbes.
