Methodological study of VITEK-MS assisted by separation gel tube for fast identification of positive blood culture bacteria
10.3969/j.issn.1673-4130.2016.15.008
- VernacularTitle:分离胶辅助VITEK-MS质谱仪快速鉴定血培养阳性菌的方法学研究
- Author:
Yueting JIANG
;
Jiancheng LI
;
Jianyu YI
;
Dingqiang CHEN
- Publication Type:Journal Article
- Keywords:
separation gel tube;
VITEK-MS;
blood culture
- From:
International Journal of Laboratory Medicine
2016;37(15):2071-2073
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the method of the separation gel vacuum tube combined with matrix‐assisted laser desorp‐tion/ionization time of flight mass spectrometry system (VITEK‐MS) for fasting identifying positive blood culture bacteria .Methods Fifty cases of positive blood culture by the BacT/ALERT3D blood culture system and Gram‐negative bacteria by direct staining in the First Affiliated Hospital of Guangzhou University from March to October 2015 were collected .The bacteria were directly ex‐tracted from the blood culture bottle by the separation gel tube and performed the fast identification by adopting the VITEK‐MS system .At the same time the bacteria were performed the subcultivation and identification .then the coincidence of this method was compared .Results Among 50 cases of Gram‐negative bacteria by positive blood culture ,21 cases of bacteria were not identified ,29 cases of bacteria were identified ,the positive rate was 58 .0% .the coincidence rate with the conventional identification results was 96 .6% ;the method of separation gel combined with VITEK‐MS was nearly 24 h in advance compared with the traditional method . Conclusion Adopting the separation gel vacuum tube combined with VITEK‐MS for identifying bacteria has the higher coincidence rate of positive blood culture Gram negative bacteria ,can greatly shorten the identification time ,this method is rapid and simple .
