Research on rapid detection of Acinetobacter baumannii produced carbapenemase by CarbaNP method
10.3969/j.issn.1673-4130.2016.15.010
- VernacularTitle:Carba NP法快速检测鲍曼不动杆菌碳青霉烯酶的研究
- Author:
Yongwei JING
;
Fangxi HU
;
Qi YAN
;
Mengqi XIA
;
Dongyue WANG
;
Xin LIU
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
drug-resistant gene;
carbapenemase
- From:
International Journal of Laboratory Medicine
2016;37(15):2076-2078
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the phenotype and enzyme genotype of pan‐drug resistant carbapenemase‐producing Acine‐tobacter baumannii to provide the evidence for clinical rational use of antibiotics and monitoring hospital infection .Methods A total of 117 clinically isolated strains of Acinetobacter baumannii were collected and performed the routine microbiological detection . Multi‐drug resistant Acinetobacter baumannii was screened by K‐B disk diffusion method .The phenotype of carbapenemase‐produ‐cing strains was detected by using the Carba NP colorimetry and modified Hodge test .The drug resistant genotype of multi‐drug re‐sistant Acinetobacter baumannii was verified by PCR .Results Among clinically isolated 117 strains of Acinetobacter baumannii ,64 strains were multi‐drug resistant Acinetobacter baumannii ,in which 33 strains were carbapenemase positive .OXA‐23 drug‐resistant genotype of carbapenemase was detected by PCR ,while IMP ,VIM and NDM‐1 drug resistant genes were not detected .Conclusion The CarbaNP method can rapidly detect carbapenemase‐producing strains with the advantages of strong sensitivity and simple oper‐ation ,which conduces to improve the detection rate of carbapenemase‐producing strains and monitor the nosocomial infection .
