Difference of peripheral blood leucocyte percentages in malaria patients among Sysmex-XE5000,CellaVisionDM96 and manual microscope classification
10.3969/j.issn.1673-4130.2016.15.012
- VernacularTitle:Sysmex-XE5000、CellaVisionDM 96及人工显微镜分类疟疾患者外周血白细胞百分比的差异
- Author:
Zhiping HOU
;
Qiuping ZHANG
;
Feidi YE
;
Zhongming ZHANG
;
Wenping ZHANG
- Publication Type:Journal Article
- Keywords:
malaria;
peripheral blood smear;
blood cell morphology;
white blood cell classification;
percentage
- From:
International Journal of Laboratory Medicine
2016;37(15):2081-2083
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the difference of peripheral blood leucocyte percentages in malaria patients among Sysmex‐XE5000 ,CellaVisionDM96 and manual microscope classification ,and to verify the accuracy and reliability of the Sysmex‐XE5000 automatic blood corpuscle detection instrument for detecting the leukocyte classification in blood routine .Methods The leucocyte percentages data in 82 cases of malaria detected by using the Sysmex‐XE5000 in the Shenzhen Municipal Third People′s Hospital from January 2011 to December 2015 were retrospectively collected ;the peripheral blood smear in 82 cases of malaria obtained the percentages after the classification by the CellaVisionDM 96 ;then the peripheral blood smear was performed the leucocyte classifica‐tion by the manual microscopy for calculating the percentage .Results In the pairwise comparison of percentage obtained from the peripheral blood leucocyte classification by Sysmex‐XE5000 ,CellaVisionDM96 and manual microscopy ,only the monocytes percent‐age had statistical difference between CellaVisionDM 96 and manual microscopy (P<0 .05) ,the other differences had no statistical significance (P>0 .05) .Conclusion By comparing the peripheral blood leucocyte percentages data in malaria patients by Sysmex‐XE5000 ,CellaVisionDM96 and manual microscopic classification ,it is indicated that the leukocyte classification data by Sysmex‐XE5000 are accurate and reliable ,malaria parasite does not affect peripheral blood leukocyte classification ,but it is necessary to pay more attention to monocytes classification in CellaVision DM 96 classification .
