Anti-proliferation effect of resveratrol and p38 MAPK in human colon cancer cells
10.3969/j.issn.1001-1978.2016.08.016
- VernacularTitle:白藜芦醇抑制人结肠癌细胞增殖与 p38 MAPK 的关系研究
- Author:
Qianzhao CHEN
;
Yuhua ZENG
;
Ying SHAO
;
Yang LI
;
Wenyan REN
;
Linyun ZHOU
;
Yi ZHOU
;
Rongxing LIU
;
Baicheng HE
- Publication Type:Journal Article
- Keywords:
resveratrol;
colon cancer;
LoVo cells;
an-ti-proliferation;
apoptosis;
p38 MAPK
- From:
Chinese Pharmacological Bulletin
2016;32(8):1110-1114
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the relationship be-tween the anti-proliferation effect of resveratrol ( Res ) and p38 MAPK in colon cancer cells .Methods Crys-tal violet staining , Western blot and flow cytometry were employed to analyze the effect of Res on the pro-liferation in LoVo cells.Western blot assay was used to detect the effect of Res on the apoptosis of LoVo cells and the phosphorylation of p 38 MAPK.Crystal violet staining and Western blot assay were used to analyze whether p38 MAPK was involved in the Res-induced proliferation inhibition and apoptosis in LoVo cells .Re-sults Res inhibited the proliferation , arrested cell cy-cle at S phase , and increased the protein level of PC-NA in LoVo cells apparently .Res increased the level of Bad in LoVo cells, but decreased the level of Bcl-2. Although Res exerted no substantial effects on total lev-el of p38 MAPK, it markedly increased the phospho-rylation level of p38 MAPK in LoVo cells.p38 MAPK inhibitor promoted the proliferation , and decreased the anti-proliferation effect of Res on LoVo cells .Moreo-ver , the effects of Res on the level of Bcl-2 and Bad were both reduced by the p 38 MAPK inhibitor .Con-clusions Res can inhibit the proliferation of LoVo cells, which may be partly mediated by promoting the phosphorylation of p38 MAPK.
