MicroRNA-506 inhibits malignancy of colorectal carcinoma cells by targeting LAMC1
10.3969/j.issn.1006-5725.2016.14.009
- VernacularTitle:microRNA-506通过调控层粘连蛋白γ1抑制结肠癌细胞的恶性表型
- Author:
Caihua ZU
;
Tao LIU
;
Guoliang ZHANG
- Publication Type:Journal Article
- Keywords:
colorectal carcinoma;
miR-506;
LAMC1;
migration;
invasion
- From:
The Journal of Practical Medicine
2016;32(14):2279-2283
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of microRNA-506 (miR-506) on malignant phenotype of colorectal carcinoma cells. To identify the target gene of miR-506 in colon carcinoma. Methods SW480 cells were divided into five groups, known as normal cell group, miR-506 overexpression and, miR-506 inhibition groups with their vehicle groups.The migration and invasion abilities of SW480 cells were measured with Transwell migration assay. Cell viability and colony forming activities were measured by CCK8 and colony formation assays, respectively. Furthermore, bioinformatic method, green fluorescent protein (GFP) reporter assays, quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were applied to predict potential target genes of miR-506. Results The number of migrated and invasive cells, viability and clonality in the miR-506 overexpression groups reduced. LAMC1 mRNA and protein levels in the miR-506 overexpression groups were lower than those in the control groups. Conclusion LAMC1 is a direct target gene for miR-506 and miR-506 could inhibit the cell migratioin and invasion.
