Enhanced activation of PERK-ATF4 pathway by Brefeldin A and cisplatin in human lung cancer GLC-82 ;cells

VernacularTitle:布雷菲德菌素A联合顺铂增强肺癌GLC-82细胞PERK-ATF4通路的激活水平
Author: Mingsong WU ; Xiang ZHENG ; Nana GENG ; Zhimin ZHANG ; Yanyu ZHAO ; Zhe WANG ; Xueying LI
Publication Type:Journal Article
Keywords: Lung cancer; PERK; ATF4; Endoplasmic reticulum stress; Brefeldin A; CDDP
From: The Journal of Practical Medicine 2016;32(14):2302-2305
CountryChina
Language:Chinese
Abstract: Objective To investigate the molecular mechanisms of synergistic effects of BFA and CDDP on human lung cancer GLC-82 cells, and to test the levels of PERK-ATF4 pathway. Methods GLC-82 cells were incubated with 50 ng/mL of BFA or/and 2 μg/mL of CDDP for 24 or 48 hours. The levels of PERK, p-PERK and ATF4 in GLC-82 were analyzed by real-time PCRand/or Western Blot. Results The levels of PERK were lowest in CDDP group, but higher in BFA group (P < 0.05), the highest in group of BFA+CDDP (P < 0.05 or P < 0.01). The p-PERK level decreased in group of BFA+CDDP (P < 0.05 or P < 0.01). There was no significant change of ATF4 expression in CDDP group, but ATF4 expression increased slightly in BFA group, and increased further in group of BFA+CDDP (P < 0.05 or P < 0.01)which was also higher than that in BFA group or CDDP group (P < 0.05 or P < 0.01). Conclusions The upregulated levels of PERK and ATF4 by the combination of BFA and CDDP may be one of the mechanisms of synergistic anti-cancer effect of BFA and CDDP on GLC-82 cells.