Effect of dexmedetomidine on apoptosis of hippocampal neurons in neonatal rat induced by propofol
10.3969/j.issn.1006-5725.2016.14.016
- VernacularTitle:右美托咪定对异丙酚诱导新生大鼠离体海马神经元凋亡的影响
- Author:
Jian YANG
;
Tingting LIU
;
Xinglong XIONG
;
Li YAN
- Publication Type:Journal Article
- Keywords:
Dexmedetomidine;
Propofol;
Neonatal rats;
Hippocampal neuron;
Apoptosis
- From:
The Journal of Practical Medicine
2016;32(14):2306-2309
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different concentrations of dexmedetomidine on the apoptosis of hippocampal neurons in neonatal rat induced by propofol in vitro. Methods Hippocampal neurons of primary cultured neonatal SD rat were divided randomly into three groups. Group C (control group)was normal cultured without any treatment for 12 h; group P (Propofol group)was incubated with 12 μg/mL propofol for 12 h and group DP (Dexmedetomidine + propofol group)was incubated with 0.002 5 ~ 25 μg/mL dexmedetomidine for 30 min, and then further incubated with 12 μg/mL propofol for 12 h. Results Compared with that of group C, the apoptosis rate of hippocampal neurons increased in group P and DP (P < 0.05 or P < 0.01); Compared with that of group P, the apoptosis rate of neurons decreased with the increase of dexmedetomidine concentration in group DP (P<0.05 or P<0.01). The result of transmission electron microscope indicated that compared with group C , group P showed obvious neuronal damage; the nerve cells damage alleviated in group DP, which were negatively associated with the concentration. Conclusions With the concentration ranging from 0.002 5 to 25 μg/mL, dexmedetomidine set pre-incubation and breeding can reduce apoptosis of hippocampus neuron of neonatal rats induced by propofol and the effect is concentration dependent.
