Effect of high molecular weight hyaluronic acid on inflammatory cytokines and chemokines of synoviocytes isolated from SD mouse
10.3969/j.issn.1006-5725.2016.13.012
- VernacularTitle:高分子量玻尿酸对大鼠膝骨关节炎滑膜细胞炎症因子和趋化因子的影响
- Author:
Yongliang YE
;
Guangwei WANG
;
Liwei HUO
;
Yongguang YE
- Publication Type:Journal Article
- Keywords:
Hyaluronic acid;
Osteoarthritis;
Inflammatory markers;
Chemokines
- From:
The Journal of Practical Medicine
2016;32(13):2116-2119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether HA affects the inflammatory cytokines and chemokines of synoviocytes. Methods FLS were derived from the SD mice with knee OA by papaya enzyme and stimulated by IL-1. Then, FLS were cultured with 600 ~ 800 kDa HA. Anti-CD44 blocking experiments were determined. The expressionsof TNF-γ, IL-1 , IL-6 , MMP-3 , MMP-9 , CX3CL1 , CCL11 , CCL2 , CXCL12 were detected by RT-PCR. In addition, the tunel test was used to detect the apoptosis rate. Results MMP-3, CX3CL1 and CCL2 gene expression were down regulated by HMW-HA in unstimulated FLS and TNF-α,MMP-3, CX3CL1 and CCL2 expression were decreased by HMW-HA in IL-1-stimulated FLS. CD44 blocking inhibited the down-regulatory effects of HMW-HA. Besides, HMW-HA promoted cell apoptosis under condition of inflammation. Conclusion HMW-HA has an anti-inflammatory effect by down-regulating of pro-inflammatory cytokines and chemokines and promoting apoptosis, possibly through the interaction of CD44 and HMW-HA.
