Platelet derived growth factor down-regulated sarcoplasmic reticulum Ca2+atpase to promote the phenotype modulation of human airway smooth muscle cells
10.3969/j.issn.1006-5725.2016.13.017
- VernacularTitle:血小板源性生长因子下调肌浆网Ca2＋-ATP酶促进人气道平滑肌细胞表型转化
- Author:
Xiaohong ZHONG
;
Yuanxiong CHENG
;
Zhihui YAO
;
Yalu YUAN
;
Jing CAO
;
Wenyan LAI
;
Kaican CAI
- Publication Type:Journal Article
- Keywords:
Human airway smooth muscle cells;
Cell phenotype;
Sarcoplasmic reticulum Ca2+atpase;
ERK pathway
- From:
The Journal of Practical Medicine
2016;32(13):2133-2137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate of the effect and mechanisman of SERCA2 on the phenotype modulation of HASMCs. Methods HASMCs were starved for 5 days and divided into different groups ,then we observed morphology change of the cells from the microscope and detected a-actin、SERCA2 and p-ERK by Western Blot,cells proliferation was observed by CCK-8 method. Results Compared with the control group,PDGF could reduce a-actin of HASMCs and increased the cells proliferation ability ,TSG could significantly inhibit the effect (P<0.01), PDGF could also significantly inhibit SERCA2 protein and increased the expression p-ERK (P<0.01), while U0126 significantly inhibited the effect (P < 0.01). Conclusion PDGF may induce HASMCs phenotype modulation through the regulation of SERCA2 and p-ERK.
