Protective effects of 10-HDA combined with AA-2G on UVA-induced photodamage in fibroblasts
10.3969/j.issn.1006-5725.2016.14.014
- VernacularTitle:10-HDA联合AA-2G对成纤维细胞光损伤的保护作用
- Author:
Jinfen ZHENG
;
Chun LU
;
Wei LAI
- Publication Type:Journal Article
- Keywords:
10-HDA;
AA-2G;
UVA;
Fibroblasts
- From:
The Journal of Practical Medicine
2016;32(14):2298-2302
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effects of 10-HDA combined with AA-2G on UVA-induced photodamage in fibroblasts. Methods The primary cultured human skin fibroblasts were divided into three groups: blank control group, UVA irradiation group and 10-HDA+AA-2G group. The cell viability and cellular senescent state were analyzed using CCK-8 and senescence associated-β-galactosidase (SA-β-gal) staining, respectively. Fluorometric assays were performed to detect the formation of reactive oxygen species (ROS) in the cells. Results Compared with those in control group, the cell viability was decreased (P < 0.05), and the SA-β-gal positive cells and ROS level were significantly increased (P<0.01 for both) in UVA-irradiated group. Compared with those in UVA-irradiated group, the cell viability was significantly increased (P < 0.01), and the SA-β-gal positive cells and ROS level were significantly reduced (P < 0.05, P < 0.01, respectively) in the 10-HDA combined with AA-2G group. Conclusion Fibroblasts treated with 10-HDA and AA-2G are significantly protected from UVA-induced cytotoxicity, cellular senescence and ROS, indicating that 10-HDA combined with AA-2G has photoprotective effects. Therefore, 10-HDA combined with AA-2G may be a potential combination for the prevention and treatment of skin photoaging.
