Inhibitory effect of 15-oxospiramilactone on the xenograft growth of ACHN human renal carcinoma cells in nude mice
10.16571/j.cnki.1008-8199.2016.08.005
- VernacularTitle:15-氧代绣线菊内酯对人肾细胞癌 ACHN 细胞裸鼠移植瘤生长的影响
- Author:
Tianyi SHEN
;
Xiaoming YI
;
Chaopeng TANG
;
Wenquan ZHOU
- Publication Type:Journal Article
- Keywords:
15-oxospiramilactone;
Renal cell carcinoma;
Wnt signaling;
Xenograft tumor
- From:
Journal of Medical Postgraduates
2016;29(8):808-811
- CountryChina
- Language:Chinese
-
Abstract:
Objective Renal cell carcinoma ( RCC) is a common renal malignancy, which is resistant to nearly all chemo-therapeutics and radiotherapy.Wnt signaling plays an important role in the tumorigenesis and cell proliferation and apoptosis of RCC. This study was to explore the inhibiting effect of 15-oxospiramilactone NC043, a new Wnt molecule inhibiter, on the xenograft growth of human renal carcinoma (ACHN) cells in nude mice. Methods ACHN cells (1 ×107) were suspended in 100μL PBS and injec-ted subcutaneously into the right side of the posterior flank of female BALB/c athymic nude mice to establish a xenograft model.The nude mice bearing ACHN cells were randomly divided into three groups, negative control, low-dose medication, and high-dose medica-tion, treated by daily intraperitoneal injection of 3%DMSO, NC043 at 45μg/kg, and NC043 at 90μg/kg, respectively, for 15 days. At 16 days, all the mice were killed, the body weight and tumor volume obtained, and the expressions of ki67, TCF4 and β-catenin determined by immunohistochemistry. Results NC043 significantly inhibited the growth of the xenograft tumor, with an inhibition rate of 36.4%in the 45 ug/kg group and 56.4% in 90 μg/kg group.The expressions of ki67, TCF4, andβ-catenin were markedly down-regu-lated in a dose-dependent manner ( P <0.01 ) . Conclusion NC043 can effectively suppress the growth of ACHN cells in the xeno-graft tumor and reduce the expression of Wnt-related proteins, andtherefore is a potential compound for the treatment of renal cell carcinoma.
