Effect of bone marrow mononuclear cells transplantation on HMGB1 migration in stroke mouse
10.3969/j.issn.1006-5725.2016.13.001
- VernacularTitle:骨髓单个核细胞移植对脑梗死小鼠神经元高迁移率蛋白1迁移的影响
- Author:
Xiaobing CUI
;
Jianping WANG
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
BMMNCs;
HMGB1;
Inflammation
- From:
The Journal of Practical Medicine
2016;32(13):2075-2078
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of bone marrow mononuclear cells (BMMNCs) transplantation on high mobility group box1 (HMGB1) migration in stroke mouse. Methods BMMNCs were harvest and injected intravenously into stroke mouse model; modified neurological severity scores(mNSS) were tested; brain infarct vol-ume and related protein expression levels were measured, then HMGB1 migration were observed. Results mNSS score and brain infarct volume in transplantation group weresignificantly lower than vehicle group , while HMGB1 expression were significantly higher than vehicle group. The expression levels of RAGE and TNF-α in transplanta-tion group were significantly lower than vehicle group. Immunofluorescence assay showed that the release of HMGB1 in brain penumbra area of transplanted mouse was significantly less than vehicle groups. Conclusions BMMNCs transplantation could inhibit the release of HMGB1 in mouse brain.
