Effects of differential expression saliva proteins on OLP patients with Huashi Xingyu Qingre decoction treatment
10.3969/j.issn.1006-5725.2016.13.047
- VernacularTitle:化湿行瘀清热方剂对口腔扁平苔藓患者唾液中差异蛋白的影响
- Author:
Tiejun LIU
;
Yangyang ZHANG
;
Jian LIU
;
Meijie ZHANG
;
Yanzhi XU
- Publication Type:Journal Article
- Keywords:
Oral lichen planus;
Saliva;
Proteomics;
2D-DIGE;
Liquid chromatography-mass spectrometry;
Western-blot
- From:
The Journal of Practical Medicine
2016;32(13):2231-2235
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effect of Huashi Xingyu Qingre decoction therapy through identification of the differentially expressed saliva proteins of oral lichen planus. Method The saliva of OLP patients before and after treatment were collected. Total saliva proteins were extracted. The differentially expressed saliva proteins were screened by two-dimensional fluorescence difference gel electrophoresis and identified by liquid chromatography-mass spectrometry. The differentially expressed proteins were analyzed by Western-blot. Results Six differentially expressed proteins were identified as salivary amylase, serum albumin, IgM, carbonic anhydrase VI, zinc-α2- glycoprote and sIgA. The expression level of serum albumin, IgM, carbonic anhydrase VI and zinc-α2-glycoprotein after treatment were lower than that before. However, the expression level of sIgA was higher. The differences were statistically significant. Conclusions Some differentially expressed saliva proteins of OLP before and after Huashi Xingyu Qingre decoction therapy are characterized, and they may play a vital part in the occurrence and development of OLP.
