Gas Chromatographic Fingerprint of Oleic Acid of Whitmania pigra Whitman
10.13359/j.cnki.gzxbtcm.2016.05.022
- VernacularTitle:宽体金线蛭油酸的气相色谱指纹图谱
- Author:
Yong HUANG
;
Zhili ZUO
;
Yilin YANG
;
Yueyun ZHANG
;
Fan WEI
;
Chengjian ZHAO
;
Jianhua MIAO
- Publication Type:Journal Article
- Keywords:
Hirudo;
gas chromatography;
fingerprints
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2016;33(5):716-719
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the gas chromatographic(GC) fingerprint of oleic acid of Whitmania pigra Whitman for its quality control. Methods Ten batches of Whitmania pigra from different sources and processed by different methods were analyzed with Agilent 6890N gas chromatography detector on DB-WAX(30 mm × 0.32 mm × 0.25μm)column at the vaporizing temperature of 270℃, column temperature of 130℃and flame ionization detector (FID) temperature of 280℃. We used a software of Similarity Evaluation System for Chromatographic Fingerprint of TCM(Version of 2004A) published by Chinese Pharmacopoeia Commission to calculate GC similarity. Results Oleic acid content of Whitmania pigra processed by different methods had significant differences (F2,7 = 7.350, P = 0.019). The oleic acid content of samples dried after washing with clean water significantly differed from that of the samples processed by alumen or the slices dried naturally(P = 0.021, P= 0.009). The similarity of the fingerprints was in the range of 0.458 - 0.998. The similarity of samples from Lipu of Guangxi Province was the lowest. Conclusion The fingerprints of most of the samples have very high similarity. The established GC fingerprints can be used to effectively identify the qualified or inferior Whitmania pigra products, which will provide some reference for the quality control of Whitmania pigra.
