Detection of TCRγ gene rearrangements in skin lesions and peripheral blood of patients with parapsoriasis ;and their clinical significance
10.3760/cma.j.issn.0412-4030.2016.08.010
- VernacularTitle:副银屑病皮损及外周血TCRγ基因重排的检测及其临床意义探讨
- Author:
Xiaoming WANG
;
Feng XUE
;
Jie ZHENG
- Publication Type:Journal Article
- Keywords:
Parapsoriasis;
Gene rearrangement,gamma-chain T-cell antigen receptor;
Mycosis fungoides
- From:
Chinese Journal of Dermatology
2016;49(8):568-572
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect TCRγ gene rearrangements in skin lesions and peripheral blood of patients with parapsoriasis, and to study their clinical significance. Methods Totally, 20 patients with parapsoriasis were included in this study. BIOMED?2 multiplex PCR was performed to detect TCRγgene rearrangements in lesional skin (n=20)and peripheral blood(n=11)samples from the patients with parapsoriasis. Statistical analysis was performed to assess the relationship of TCRγ gene rearrangements with clinical types of parapsoriasis as well as general information and histopathological manifestations(including non?specific manifestations and atypical manifestations)of patients. Results TCRγ gene rearrangements were positive in lesional skin from 7 of the 20 patients, in peripheral blood from 3 of 11 patients, and in both lesional skin and peripheral blood from 2 patients. Positive TCRγ gene rearrangements in skin lesions were significantly correlated with mycosis fungoides(MF)?related atypical histopatho?logical manifestations(P<0.05), but those in neither skin lesions nor peripheral blood were correlated with gender and age of patients or clinical course and types of parapsoriasis(all P>0.05). During an average follow?up time of 44.85 ± 18.48 months, 1 case progressed into MF, and 2 were cured. Conclusions Positive TCRγgene rearrangements in skin lesions of patients with parapsoriasis may be correlated with MF?related atypical manifestations. The presence of TCRγgene rearrangements and atypical histopathological manifestations may suggest the possibility of progression from parapsoriasis into MF.
