Effects of bone marrow-derived mesenchymal stem cells on glomerular podocyte injured by lipopolysaccharide

VernacularTitle:骨髓间充质干细胞对脂多糖诱导的足细胞损伤的影响
Author: Yi CHEN ; Linfen LIU ; Jianxin WAN ; Jiong CUI ; Danyu YOU ; Zhenhuan ZOU
Publication Type:Journal Article
Keywords: Podocyte; Mesenchymal stem cells; lipopolysaccharides; Co-culture
From: Chinese Journal of Nephrology 2016;32(7):528-533
CountryChina
Language:Chinese
Abstract: Objective To observe the effects of bone marrow?derived mesenchymal stem cells (BMSC) on glomerular podocyte injured by lipopolysaccharide (LPS) and the expression of related protein. Methods Podocytes are divided into control group, BMSC group, LPS group and LPS plus BMSC group. After 24 hours of intervention, observing each experimental group podocyte form under inverted phase contrast microscope;detecting the expressions of mRNA and protein of nephrin, CD2AP, synaptopodin, and TRPC6 by RT?PCR and Western?blot. Results Compared with control group, expressions of nephrin, CD2AP, and synaptopodin in LPS group decreased (P<0.05) while that of TRPC6 increased (P<0.05); compared with LPS group, expressions of nephrin, CD2AP, and synaptopodin in LPS+MSC group increased (P<0.05) while that of TRPC6 decreased (P<0.05). Conclusion BMSC may relieve LPS?induced podocyte injury.