The relation between epithelial-mesenchymal transition and bronchiolitis obliterans post lung transplantation in rats
10.3760/cma.j.issn.1001-4497.2016.07.008
- VernacularTitle:大鼠上皮－间质转化和肺移植后闭塞性细支气管炎的相关性
- Author:
Chong ZHANG
;
Li YU
;
Wang LYU
;
Haichao XU
;
Abudumailamu ABUDUWUFUER
;
Jinlin CAO
;
Jian HU
- Publication Type:Journal Article
- Keywords:
Epithelial-mesenchymal transition;
Lung transplantation;
Chronic rejection;
Bronchiolitis oblit-erans;
Vimentin;
E-cadherin
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2016;32(7):411-414
- CountryChina
- Language:Chinese
-
Abstract:
Objective To imitate the pathological process of bronchiolitis obliterans(BO) post lung transplantation and in-vestigate the phenomenon of epithelial-mesenchymal transition ( EMT ) in small airway epithelial cells and its relation to BO . Methods We established the rat model of allogeneic orthotropic left lung transplantation with grafts from F 344 rats transplanted to Lewis rats by the Tri-cuff anastomosis.Then rats were daily injected intraperitoneally with cyclosporine (5 mg· kg-1 · d-1 ) for 10 days, intratracheally applied with lipopolysaccharide(0.5 mg/kg) at the 28th day and killed at the 90th day post-surger-y.We observed the histological structure of the lung grafts by HE and Masson staining , detected E-cadherin and Vimentin ex-pression in lung grafts and normal ones of F344 rats by immunohistochemistry, then analyzed the relation between small airway remodeling and the alterations in EMT markers expression .Enumeration data was analyzed with Fisher exact test and Spearman rank correlation was applied in correlation analysis .Results We found inflammatory cell infiltration , fibroplasia of bronchiole walls and significant lumen stenosis in lung graft mesenchyma.In lung grafts, the positive expression of E-cadherin was 5(total 13), which was significantly lower than that in normal lungs(7/8, P<0.05), and conversely the positive expression of Vim-entin was higher than normal ones(10/13 vs 2/8, P <0.05).The Spearman rank correlation analysis demonstrated that E-cadherin expression bear a negative correlation with Vimentin expression(r=-0.750, P<0.01).Conclusion Our study established a disease model imitating the pathological process of BO .And EMT was observed in small airway epithelial cells of the BO-exhibited lung grafts, indicating that EMT was involved in the process of BO airway remodeling .Thus it may reveal no-vel therapeutic targets for BO post lung transplantation by further investigation into the mechanism and pathological significance of EMT in small airway epithelial cells.
