Expression and significance of human telomerase reverse transcriptase mRNA in different subtypes of melanoma
10.3760/cma.j.issn.1006-9801.2016.07.002
- VernacularTitle:人端粒酶反转录酶mRNA在不同类型黑素瘤中的表达及其意义
- Author:
Mingfeng ZENG
;
Xiaojing KANG
;
Ying ZENG
;
Li CHAI
;
Xiujuan WU
;
Juan ZHAO
;
Zhenzhu SUN
;
Ying WANG
;
Weijia WANG
- Publication Type:Journal Article
- Keywords:
Melanoma;
Mucous membrane;
Human telomerase reverse transcriptase;
Real-time fluorescence quantitative PCR
- From:
Cancer Research and Clinic
2016;28(7):437-440
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of human telomerase reverse transcriptase (hTERT) mRNA in the melanoma, and to analyze the relationship between the expression and subtypes and clinicopathological features of melanoma. Methods Expression of hTERT mRNA was detected by real-time quantitative PCR in 64 cases of melanoma and 30 cases of nevus. SPSS 17.0 software was used to analyze the relationship between hTERT mRNA expression and clinical pathological features of melanoma. Results The relative expression of hTERT mRNA in melanoma tissues was higher than that in nevus tissues [(52.43±5.42) vs (21.38±3.73), t= 4.72, P= 0.000]. The expression of hTERT mRNA in melanoma had no significant correlation with age, gender, ethnicity (all P> 0.05), but had relationship with subtypes, lymph node metastasis, Clark classification (all P< 0.05). The expression of hTERT mRNA in mucosal melanoma was significantly higher than that of acral and non-acral melanoma (t= 7.71, P= 0.001), while the expression of acral and non-acral melanoma had no difference (P> 0.05). Conclusions The expression of hTERT mRNA in melanoma is high, especially in mucosal melanoma. hTERT may play an important role in the occurrence and development of melanoma.
