Spatiotemporal expression spectrum of Rap1 gene in zebrafish early development process
10.3969/j.issn.1671-8348.2016.20.004
- VernacularTitle:Rap1基因在斑马鱼胚胎早期发育过程中的时空表达谱研究
- Author:
Xiaoyan YANG
;
Zhixu HE
;
Liping SHU
;
Jiao JIN
;
Jing HUANG
;
Sasa WU
;
Jianjuan MA
- Publication Type:Journal Article
- Keywords:
Rap1 gene;
zebrafish;
in situ hybridization;
gene expression
- From:
Chongqing Medicine
2016;45(20):2748-2751
- CountryChina
- Language:Chinese
-
Abstract:
Objective To choose zebrafish as the experimental animal model for studying the spatiotemporal expression rule of rap1 gen in zebrafish embryo early development process .Methods The Rap1 gene fragment was cloned from the zebrafish emby‐oscDNA ,then the Rap1 gene fragment and pCS2+ plasmid were performed the in vitro connetion and recombination was extracted , the combinant plasmid was correct after the double enzyme digestion ,colony PCR and sequencing identification .T3 RNA polymer‐ase in vitro transcription system was used to obtain the digoxin (DIG)‐labeled anti‐sense mRNA probe of Rap1 gene .The whole mount in situ hybridization method was adopted to detect the Rap1 expression in zebrafish embryo early development process . Results The positive hybridization signal of Rap1 gene was detected at the cell division junction region of 0 .75 hpf ,animal pole of 3 .70 hpf and 6 .00 hpf ,and notochord of 12 .00 -72 .00 hpf .Conclusion Rap1 gene might be involved in the early development process of notochord nervous system in zebrafish .
