Analysis of biofilm formation ability and related genes of Staphylococcus aureus isolated from urinary tract infections
10.3969/j.issn.1671-8348.2016.19.008
- VernacularTitle:尿路感染分离金黄色葡萄球菌生物膜形成能力及相关基因的分析
- Author:
Fengjun SUN
;
Hai HONG
;
Wei FENG
;
Yixuan SUN
;
Peiyuan XIA
- Publication Type:Journal Article
- Keywords:
Staphylococcus aureus;
urinary tract infections;
biofilm;
adhesion;
ica gene
- From:
Chongqing Medicine
2016;45(19):2617-2619
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biofilm formation ability and related gene distribution of Staphylococcus (S .) aureus iso‐lated from urinary tract infections to provide the theoretical basis for the prevention and treatment of clinical infection .Methods The minimal inhibitory concentration was detected using the agar double dilution method .The bacterial adhesion ability was deter‐mined by flat colony counting method .The biofilm formation ability was analyzed by the 96‐well crystal violet staining method .The biofilm‐associated genes were detected by PCR amplification .Results Eleven clinical strains of S .aureus were high resistant to pen‐icillin and erythromycin ,whereas were all sensitive to vancomycin and nitrofurantoin .All the isolates had a strong ability of adhe‐sion ,but the biofilm formation ability was weak .Among them ,the icaAD and icaBC genes were amplified in 10 S .aureus isolates . Conclusion The adhesion ability and biofilm formation ability of S .aureus isolated from urinary tract infections have the strain differences ,and ica is an important gene of S .aureus biofilm formation .
