IGF-II gene promoter P3 methylation in salivary pleomorphic adenoma
10.3969/j.issn.1001-3733.2016.03.014
- VernacularTitle:胰岛素样生长因子Ⅱ基因启动子 P3甲基化状态与涎腺多形性腺瘤发生的关系
- Author:
Li LIU
;
Hong MA
- Publication Type:Journal Article
- Keywords:
Salivary pleomorphic adenoma(SPA);
Insulin-like growth factor Ⅱ(IGF-II);
Promoter P3 of IGF-II;
Methylation;
Nested methylation specific polymerase chain reaction;
Pyrosequencing
- From:
Journal of Practical Stomatology
2016;32(4):517-521
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the methylation status of insulin-like growth factor Ⅱ(IGF-Ⅱ)gene promoter P3 in salivary pleo-morphic adenoma(SPA).Methods:The methylation of IGF-Ⅱ gene promtor P3 was examined in 26 cases of salivary pleomorphic adenoma with adjacent normal salivary gland tissues,1 0 cases of salivary gland malignant tumor (except malignant pleomorphic ade-noma)and 1 0 cases of normal salivary gland tissue by nested methylation specific polymerase chainreaction(nMSP),1 0 samples(3 SPA and controls,2 malignance and controls)were examined by pyrosequencing.Results:9 out off the 26 SPA showed lower level methylation oevel of IGF-II gene promoter P3.Normal salivary gland and salivary gland malignant tumor tissue did not.Conclusion:IGF-II gene promoter P3 with low level of methylation may play a role in the development of SPA.
