The effects of PRF and its three growth factors on the proliferation and adhesion of rat ADSCs
10.3969/j.issn.1001-3733.2016.04.003
- VernacularTitle:PRF 及所含三因子对大鼠脂肪干细胞增殖和黏附的影响
- Author:
Jie GAO
;
Mingguo WANG
;
Xiumei LI
;
Shuai YANG
;
Xue LI
- Publication Type:Journal Article
- Keywords:
PRF;
ADSCs;
Proliferation;
Adhesion;
TGF-β1;
PDGF-AB;
VEGF
- From:
Journal of Practical Stomatology
2016;32(4):462-466
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of PRF and recombinant hPDGF-AB,TGF-β1 and VEGF on the proliferation and adhe-sion of rat adipose tissue-derived stem cells(ADSCs)in vitro.Methods:ADSCs were cultured with PRF membrane and various do-ses of PDGF-AB,TGF-β1 and VEGF,cell adhesion was examined by adhesion assay after 2 culture,cell proliferation was examined by CCK-8 kit after 1 -7 d culture.Results:Cell adhesion assay showed that the adhesive numbers of rat ADSCs in PRF group were significantly higher than those in the negative group(P <0.05).The adhesive numbers of the ADSCs treated by PDGF-AB showed no significantly difference among different concentration groups(P >0.05).The adhesive numbers of the ADSCs treated by VEGF or TGF-β1 at different concentrations showed significant difference(P <0.05).CCK-8 kit assay showed that at different time points, the A values of ADSCs in PRF group were significantly higher than those of the negative control group(P <0.05).The A values of ADSCs in VEGF or PDGF-AB groups at different concentrations showed significant difference(P <0.05).The A values of rat AD-SCs in TGF-β1 group at different concentrations were lower than those in the negative control group(P <0.05).Conclusion:PRF as a combination of growth factors may stimulate the proliferation and adhesion of rat ADSCs in vitro.PDGF-AB and VEGF may stim-ulate the proliferation of rat ADSCs.TGF-β1 and VEGF may stimulate the adhesion of rat ADSCs in a dose-response manner to some degree.
