The effects of HtrA on the expression and biological activity of GTFs of Streptococcus mutans isolated from children with high cario-susceptibility
10.3969/j.issn.1001-3733.2016.04.006
- VernacularTitle:HtrA 对乳牙高致龋性变异链球菌 GTFs 表达及活性的影响
- Author:
Zheng LI
;
Hongqian ZHU
;
Ru XIE
;
Feifei LI
;
Xingrong LIU
- Publication Type:Journal Article
- Keywords:
Streptococcus mutans;
High temperature requirment serine proteinase A(HtrA);
Glucosyl transferases (GTFs);
Cariogenecity
- From:
Journal of Practical Stomatology
2016;32(4):475-479
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the expression and biological activity of glucosyl transferases (GTFs)of Streptococcus mutans (S.mutans)under normal outside environment between high temperature requirment serine proteinase A(HtrA)-deficient strains and high virulent strains isolated from children with high cario-susceptibility.Methods:The HtrA-deficient strains and high virulent strains of S.mutans were obtained by preliminary study.The strains were reanimated and incubated in BHI medium to exponential phase at tenth hour.The expression of gtfB,gtfC and gtfD were detected by real-time RT-PCR.The biological activity of the GTFs were detected by entong sulfuric acid method and Western Blot.Results:The expression of gtfs and GTFs in the HtrA-deficient strains was higher than those of high virulent strains,but the biological activity of the GTFs was lower.Conclusion:The HtrA gene plays an important regulatory role in the process of the GTFs expression of S.mutans isolated from children with high cario-susceptibility.
