Gene regulatory network during the osteogenic differentiation process of dental follicle stem cells:A bioin-formatic analysis
10.3969/j.issn.1001-3733.2016.04.007
- VernacularTitle:牙囊干细胞成骨分化基因调控网络的生物信息学分析
- Author:
Rui WANG
;
Peng MA
;
Lingzhi LI
;
Jianqing GUO
- Publication Type:Journal Article
- Keywords:
Dental follicle stem cells;
Osteogenic differentiation;
Regulatory network;
Bioinformatic analysis
- From:
Journal of Practical Stomatology
2016;32(4):480-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the gene regulatory network during the osteogenic differentiation process of dental follicle stem cells (DFSCs)with bioinformatic analysis.Methods:The differentially expressed genes during the osteogenic diffrentiation process of DF-SCs were selected from the GEO Datasets.Then the relationship among the genes were analysed using DAVID and GeneMANIA data-base.Results:Numerous differentially expressed genes during the osteogenic differentiation process of DFSCs were obtained,289 genes with significance of different expression(P <0.05)were enriched into “cell differentiation”,“cell proliferation”,“skeletal sys-tem development”and “calcium signaling pathway”subgroups.ALPL,CTSK,TUFT1 ,TGFBR2,FHL2,ROR2,STC1 ,POSTN, ZBTB1 6,FRZB,PRELP and IGFBP5 were enriched into the “skeletal system development”subgroup.The 1 2 genes exhibited interac-tions,including co-expression,co-localization,genetic interaction and signal pathways.Conclusion:There is a complex molecular regulatory network among the differentially expressed genes during the osteogenic differentiation process of DFSCs.It is necessary to an-alyse the osteogenic differentiation process of DFSCs as a whole from WNT,TGFbeta,MAPK siganal pathyways,Homeobox transcrip-tion factors and osteogenic differentiation marker genes.
