The effects of erythropoietin on the proliferation and osteogenic differentiation of human dental pulp cells
10.3969/j.issn.1001-3733.2016.04.008
- VernacularTitle:促红细胞生成素对人牙髓细胞增殖和成骨分化的影响
- Author:
Wei HAN
- Publication Type:Journal Article
- Keywords:
Erythropoietin;
Dental pulp cells;
Proliferation;
Differentiation
- From:
Journal of Practical Stomatology
2016;32(4):485-489
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of erythropoietin (EPO)on the proliferation and osteogenic differentiation of human dental pulp cells(hDPCs)in vitro.Methods:Isolated hDPCs were cultured and identified.The cells were treated by EPO and the proliferation of the cells was determined by CCK-8 assay.After incubation with EPO at 20 U /ml in osteogenic induction medium for 7 and 1 4 days,the mineralization of hDPCs was observed by alkaline phosphatase (ALP)activity assay and alizarin red staining.Real-time PCR was utilized to detect the expression of odontogenesis-related genes.Results:EPO enhanced the proliferation of hDPCs in a time-and dose-dependent manner.After treatment with EPO,ALP activity and the minerialized nodes of the cells increased(P <0.05);the expression levels of odontogenesis-related genes DSPP,OCN,OSTERIX and RUNX2 were upregulated(P <0.05).Con-clusion:EPO can promote proliferation and differentiation of human dental pulp cells.
