Association of rs1 1 742688 polymorphism of DHFR gene with non-syndromie cleft lip with or without cleft palate in the northeast of China

VernacularTitle:DHFR基因rs11742688位点单核苷酸多态性与中国东北人群唇腭裂相关性研究
Author: Jiantao YU ; Kun LIU ; Zengjian LI ; Qiang LIU ; Yongping LU ; Huan ZHANG
Publication Type:Journal Article
Keywords: Non-syndromie cleft lip with or without cleft palate(NSCL/P); Dihyrofolate reductase(DHFR); Single-nucleotide polymorphism(SNP)
From: Journal of Practical Stomatology 2016;32(4):552-556
CountryChina
Language:Chinese
Abstract: Objective:To investigate the association between dihyrofolate reductase(DHFR)gene rs1 1 742688 polymorphism and non-syndrom cleft lip with or without cleft palate (NSCL/P)in northest Chinese population.Methods:PCR-restriction fragment length polymorphism(PCR-RFLP)was used to identify the rs1 1 742688 polymorphism of DHFR gene of 220 NSCL/P patients(inclu-ding 1 38 core families)and 1 80 healthy controls.Hardy-Weinberg test and SPSS statistical software were used to calculate the data, OR and 95% confidence intervalarents.Results:In case-contral analysis,there was no significant difference in TT genotype of rs1 1 742688 between NSCL/P subjects and the controls(χ2 =0.439,P >0.05)in.Conclusion:The polymorphism of rs1 1 742688 in DHFR gene is not associated with NSCL/P in northest Chinese population.