Analysis on etiological diagnosis of first case of imported Plasmodium ovale wallikeri subspecies in Hubei Province
10.3969/j.issn.1673-4130.2016.14.022
- VernacularTitle:湖北省首例输入性卵形疟原虫wallikeri亚种的病原学诊断分析
- Author:
Lingcong SUN
;
Huaxun ZHANG
;
Sujian PEI
;
Jing XIA
;
Dongni WU
;
Wen LIN
- Publication Type:Journal Article
- Keywords:
imported malaria;
plasomdium ovale wallikeri;
nested polymerase chain reaction
- From:
International Journal of Laboratory Medicine
2016;37(14):1956-1958
- CountryChina
- Language:Chinese
-
Abstract:
Objective To use the nested PCR technology to diagnose and identify a case of imported Plasmodium ovale wallikeri subspecies .Methods Blood sample was collected from 1 case of initially diagnosed imported tertian malaria and performed the ex‐amination of microscopy ,rapid diagnostic tests (RDTs) and nested‐PCR .Moreover the sequencing was conduced .Results RDTs showed the negative result ;the ring form and trophozoite of Plasmodium could be observed in the blood smear by microscopy ;the Plasmodium ovale wallikeri subspecies specific primer rOVA 1v/rOVA2v was adopted for conducting nested PCR ,the specicific am‐plification band appeared at 760 bp ,after sequencing and Blast aligning ,its coincidence with the partial sequence of Plasmodium ovale wallikeri subspecies in the Genbank database was 99% .Conclusion This patient is the first case of Plasmodium ovale wallik‐eri subspecies infection in Hubei province by nested PCR and sequencing analysis .
